IMAGE

Figure 1

ID

ZDB-IMAGE-250419-29

Genes

alpl

Antibodies

Ab2-alpl

Publication

Ciapaite et al., 2025 - Broad Vitamin B₆-Related Metabolic Disturbances in a Zebrafish Model of Hypophosphatasia (TNSALP-Deficiency)

All Figures

Figures for Ciapaite et al., 2025

Image

Figure Caption

Figure 1

Generation and basic characterization of alpl^-/- zebrafish line. (a) Schematic representation of vitamin B₆ metabolism. (b) Sanger sequencing results showing a 10 bp deletion at the CRISPR site in exon 5 of the alpl gene (c.623_632del, p.Gln180fs), which is predicted to result in a truncated protein. (c) Agarose electrophoresis of genotyping PCR products for the CRISPR site in exon 5 of the alpl gene in wild type (WT), alp^+/-, alpl^-/- 5 dpf old zebrafish embryos. (d) Relative alpl mRNA expression in WT, alpl^+/-, and alpl^-/- 5 dpf old zebrafish embryos. Data are means from n = 3 pools (10 embryos per pool) per genotype measured in duplicate ± SD. **** p < 0.0001. (e) Total alkaline phosphatase activity in whole-embryo extracts of WT, alpl^+/-, and alpl^-/- 5 dpf old zebrafish. RLU, relative light unit. Data are means from n = 3 pools (10 embryos per pool) per genotype ± SD. **** p < 0.0001 and *** p < 0.001. (f) Alpl protein expression in total WT and alpl^-/- 5 dpf zebrafish embryo extracts showing lack of alpl protein in alpl^-/- embryos (predicted molecular weight of alpl protein (NP_957301.2) is 62.5 kDa). Data are from pools of n = 32 embryos per genotype. Gapdh protein expression was used as the loading control.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.