Fig. 4

Fig. 4

In liver metastasis, 5hmC peaks that were initially present in normal colon are recovered. A IGV screenshot with uploaded tracks of 5hmC consensus peaks for normal colon, colon tumour and liver metastasis. Boxes above R highlight a 5hmC peak that has ‘recovered’ since it is present in normal colon, absent in colon tumours and reappears in liver metastasis. Boxes above RS highlight a 5hmC peak that has ‘recovered and spread’. Thus, the peak is present in normal colon, lost in tumour colons and reappears in metastasis, but also spreads to adjacent sites. B The strategy for identifying RS peaks and the consensus binding sites for SALL4, ZNF770, ZNF121 and PAX5 identified after SEA. Abbreviations: MLT and MNL are Merged liver tumour (LT) and normal liver (NL) peaks respectively. LTO and NLO are LT or NL peaks that Overlap normal colon. C Normalised RNAseq counts (data used [28]) for SALL4, ZNF770, ZNF121 and PAX5 in normal colon, colon tumours and liver metastasis (NC, CT and LM). Wald test, Benjamini-Hochberg adjusted. Error bars represent ± SEM. Supporting PCR data values in Additional file 6