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Fig. 4 Prep regulates the proliferation of bipotential progenitor cells. (A) Single-section confocal microscope images showing anti-PCNA and anti-2F11 antibody staining of the regenerated livers of WT siblings and Prep?/? at BT, R0h, R8h, R24h and R48h. (B) Ratio of PCNA+ cells to 2F11+ cells in regenerated livers from A. The probability of PCNA+/2F11+ in regenerating livers was significantly reduced in Prep?/? compared with WT. (C) Single-section confocal microscope images showing anti-PCNA and anti-2F11 antibody staining of the regenerated livers from the DMSO and PRA treatment groups at BT, R0h, R8h, R24h and R48h. (D) Ratio of PCNA+ cells to 2F11+ cells in regenerated livers from C. (E) Single-section confocal microscope images showing anti-PCNA and anti-2F11 antibody staining of the regenerated livers of WT siblings and Tg (hsp701: Prep) at BT, R0h, R8h, R24h and R48h. (F) Ratio of PCNA+ cells to 2F11+ cells in regenerated livers from E. ns, not significant; *P<0.05, ***P<0.001 (two-tailed post hoc test). Data are meanħs.e.m. Scale bars: 75?µm.