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Figure 8. Mounting of embryos and larvae after RNAscope reagents treatment.
Top images show Ibidi dishes, with the recommended dissection area on the top and the agarose mounting area at the bottom (white rectangles). 48 hpf embryos (a, b) and 5 dpf larvae (a’, b’) were either (a, a’) or not (b, b’) treated with proteinase K and all of them with RNAscope reagents. Images show that the whole procedure leads to virtually total transparency of the specimen, which reaches a maximum for embryos [becoming barely visible as in (a)]. This transparency requires controlling all steps of reagent/buffer removal under the binoculars. Yolks were dissected, as well as the eyes facing the glass bottom of the dish to ensure flatness.