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Figure 2. Efficiency of proteinase K digestion on the zebrafish embryo and larva.
48 hpf embryos (a) and 5 dpf larvae (b) were enzymatically digested or not (+ proteinase K, - proteinase K, respectively) after formaldehyde and methanol fixation. Note the effect of proteinase K treatment that is visualized by an increase in transparency of both the embryo and larva. In the embryo, the brain also appears more “milky,” which indicates the extreme limit of acceptable digestion.
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