Fig. 2

Fig. 2

Effect of in vivo HyPer zebrafish H₂O₂ exposure on tRF-Glu-CTC levels. A H₂O₂ levels in 72 hpf HyPer zebrafish embryos for 24 h. HyPer imaging for different concentrations of H₂O₂. The H₂O₂ levels are inferred from the F₅₀₀/F₄₂₀ excitation ratio of HyPer. Respectively, control, 1 mM, 1.5 mM, and 2 mM H₂O₂. B Quantification of the H₂O₂ levels in the control and H₂O₂ concentrations in 72 hpf HyPer zebrafish embryos; images were taken 20 × magnification, Zeiss LSM 880 confocal microscopy (data are presented as boxplot, n = 5, ns, Kruskal–Wallis test, p = 0.168). C Changes in levels of tRF-Glu-CTC fragment in HyPer 72 hpf embryos after 24 h H₂O₂ exposure (data are presented as boxplot, n = 6, Kruskal–Wallis test, *p < 0.05, **p < 0.01)