IMAGE

FIGURE 2

ID

ZDB-IMAGE-250411-4

Genes

opa1

Publication

Strachan et al., 2025 - Novel in vivo models of autosomal optic atrophy reveal conserved pathological changes in neuronal mitochondrial structure and function

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FIGURE 2

Loss of Opa1 impairs visual function in zebrafish. (A) Schematic of OKR assays which were used to assess visual function in <131 hpf larvae. No gross morphology differences are distinguished between opa1 crispants and buffer injected controls. (B–D) Visual acuity responses of <131 hpf opa1 crispants and controls to drums with 0.2 (B) and 0.02 (C) cycles per degree (cpd) and 0.2 cpd with reduced (20%) black/ white contrast (D). (E) A representative PCR genotyping electrophoresis gel from opa1^+/+, opa1^+/− and opa1^−/− larvae. opa1 mutation produces a 932 bp deletion band. No gross morphological differences were observed between opa1^−/− animals and opa1^+/+ siblings. (F) qPCR shows a significant reduction of opa1 transcript (normalized to B‐Actin) in both opa1^−/− and opa1^+/− animals. (G, H) Visual acuity responses of <131 hpf opa1^+/+, opa1^+/− and opa1^−/− larvae to drums with 0.2 (G) and 0.02 (H) cycles per degree. All graphs represent mean ± standard deviation (SD) saccades per minute. Statistical analysis consists of one‐way ANOVA and Tukey's multiple comparisons tests.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ FASEB J.