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Fig. 1 Design of targeted LNPs and their in vitro and in vivo evaluation. A) DNA-LNPs decorated with different targeting ligands: Herceptin (Her; turquoise), folate (FA; pink), and both (Dual; purple), respectively. B) Targeted LNPs were analyzed regarding their efficiency to specifically bind and transfect different breast cancer cell lines in vitro: MCF7 (yellow, low HER2 expression), MDA-mb453 (orange, intermediate HER2 expression), and SKBR3 (brown, strong HER2 expression). C) Xenograft zebrafish larvae (Danio rerio) were used to analyze the LNP targeting capacity in vivo. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)