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Fig. 3 ROS accumulation of F1 larvae (96 hpf) derived from parents exposed to 0, 25, 250, and 2500 μg/L of [C8mim]Br for eight weeks. (A): Representative larvae stained by DCFH-DA probe under a fluorescence microscope. Stronger green fluorescence represents higher ROS content. (B) and (C): Fluorescence intensity was quantified using Image Pro Plus software and microplate reader, respectively. Significant differences between the [C8mim]Br exposure groups and the control group are indicated by * (P < 0.05).
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