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Fig. S4 Analysis of SC division during radial sorting in embryos treated with aphidicolin between 22 and 40 hpf. PH3 immunolabeling at 52 hpf in Tg(foxd3:gfp) (A?A?) and Tg(foxd3:gfp) embryos treated with aphidicolin between 22 and 40 hpf (B?B?). Arrow in A? and B? indicate SCs that are GFP and PH3 positive. A?, merge of A and A?; B?, merge of B and B?. Scale bar = 20 ?m. (C) Quantification of the number of Schwann cells within a defined region of the PLLn at 52 hpf in control (average of 59.10 ± 2.44 cells, n = 10 embryos) and embryos treated with aphidicolin between 22 and 40 hpf (average of 18 ± 3.27 cells, n = 11 embryos) (????, p ? 0.0001). (D) Quantification of the number of PH3+/Schwann cells within a defined region of the PLLn at 52 hpf in control (average of 0.87 ± 0.47, n = 8 embryos) and embryos treated with aphidicolin between 22 and 40 hpf (average of 0.18 ± 0.12, n = 11 embryos) (ns, p = 0.3265). (E) Quantification of the percentage of PH3+ Schwann cells relative to the total number of Schwann cells within a defined region of the PLLn at 52 hpf in control (average of 2.54 ± 0.95, n = 10 embryos) and embryos treated with aphidicolin between 22 and 40 hpf (average of 2.34 ± 1.65, n = 11 embryos) (ns, p = 0.3274). (F) Still images of time-lapse imaging at 52 hpf in Tg(foxd3:gfp) and Tg(foxd3:gfp) treated with aphidicolin between 22 and 40 hpf embryos. Arrows indicate Schwann cells along the PLLn at different timepoints prior to and after division. Scale bars = 20 ?m. (G) Quantification of the time required for control (average of 8.70 ± 0.41 min, 20 cells, n = 5 embryos) and aphidicolin treated embryos between 22 and 40 hpf (average of 8.14 ± 0.67 min, 7 cells, n = 4 embryos) Schwann cells to successfully complete mitotic division during radial sorting. (ns, p = 0.1796).