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Fig. 7 Alteration of hyaloid vasculature in larvae treated with proteasome inhibitor, which could be rescued by proteasome activator. (A) Scheme of experimental design for hyaloid vasculature exploration in larvae treated with BOR with or without BA rescue. Figure was created with BioRender.com. (B) Representative confocal images of the hyaloid vasculature showed amplified alterations in WT, GLO1KO and ALDH3A1KO larvae treated with 7.5 μM BOR, and attenuated alterations in larvae co-treated with 7.5 μM BOR and 200 μM BA. White scale bar = 20 μm. (C) Increased hyaloid branch points in WT, GLO1KO and ALDH3A1KO larvae treated with 7.5 μM BOR was observed compared to the larvae without treatment, but no alteration was observed in DKO larvae treated with BOR, and these vasculature alterations can be rescued by 200 μM BA. n = 17–26. The bars indicate mean ± SD values. Statistical analysis was applied by one-way ANOVA, ns = not significant, ***p < 0.001. hpf, hours of post fertilization; dpf, days of post fertilization; w/o, with or without; BOR, bortezomib; BA, betulinic acid.