FIGURE 5

FIGURE 5

SB218078 inhibits the growth and angiogenesis of breast cancer in vivo. (A) Representative images of MDA-MB-231 cell xenografts treated with either vehicle control (DMSO) or SB218078. (B) Growth curve showing the progression of the xenografts, and (C) the corresponding weights of the xenografts. (D, E) Immunohistochemical (IHC) staining for Ki67 in xenograft tissues. (F, G) IHC analysis of microvessel density (CD31/mm²) between the two treatment groups in xenograft tissues. (H, I) Immunofluorescence assessment of microvessel density (CD31/mm²) in xenograft tissues for both groups. (J) Representative images depicting eGFP-expressing blood vessels (green) and mCherry-labeled MDA-MB-231 cells (red) following treatment of zebrafish embryos with vehicle control (DMSO) or SB218078 (2.5 μM) for 3 days. Scale bars: 200 µm. (K) Quantification of relative vascular density in each group. Scale bar in (D, F, H, and J) 50 μm.