Fig. 6

Fig. 6 Inhibiting lipid peroxidation improves cell adhesion and reduces podocyte permeability. (a, b, c, d) Adherence of cells incubated with 100 µM cysteamine, 10 µM liproxstatin-1, or 100 µM cysteamine?+?10 µM liproxstatin-1 for 48 h, normalized to a. CTNS?/?#1, b. CTNS?/?#2, c. shCTNS#1, and d. shCTNS#2. Each dot represents the relative number of podocytes attached in one well. (n???3 biological experiments, n???3 technical replicates). Statistical analysis: linear mixed model. (e, f, g, h) FITC-BSA fluorescence quantification of cells incubated with 100 µM cysteamine, 10 µM liproxstatin-1, or 100 µM cysteamine?+?10 µM liproxstatin-1 for 48 h, normalized to e. CTNS?/?#1, f. CTNS?/?#2, g. shCTNS#1, and h. shCTNS#2. (n???4 biological experiments, n?=?4 technical replicates). Statistical analysis: Dunnett?s One-Way ANOVA. Unless noted, each dot represents a biological experiment