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Fig. 6 IR treatment impacts gene expression in wildtype but not tp53 mutant embryos while under control conditions. Wildtype, tp53 null, R217H/R217H, and R242H/R242H mutants have largely varying DEGs and GO terms affecting metabolism, biosynthesis and developmental processes, and cell signalling. (A) Heatmap of p53 target genes that were differentially expressed between 0 and 30 Gy conditions across all genotype/treatment samples (adjusted p-value < 0.05, fold change >2). Genes that were also measured by qPCR in Fig. 2 are listed in red. (B) A heatmap of all differentially expressed genes from all genotype/treatment group comparisons (adjusted p-value < 0.05, fold change >2). (C) A heatmap showing DEGs from all genotype comparisons from the 0 Gy treatment groups (adjusted p-value < 0.05, fold change >2). (D) UpSet plot shows the number and overlap of DEGs between each genotype from the 0 Gy treatment groups. The total number of DEGs per set is shown on the x-axis and the y-axis shows the intersection of DEGs for each comparison. Comparisons with an intersection of less than ten are not shown. (E, F) Dot plots showing the top ten GO BP (E) and KEGG pathway terms (F), ranked by combined score, for each comparison under control conditions. Dot size corresponds to the ratio of DEGs per total number of genes for each term and the colour corresponds to the combined score. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Reprinted from Biochimica et biophysica acta. Molecular basis of disease, 1871, Kobar, K., Tuzi, L., Fiene, J.A., Burnley, E., Galpin, K.J.C., Midgen, C., Laverty, B., Subasri, V., Wen, T.T., Hirst, M., Moksa, M., Carles, A., Cao, Q., Shlien, A., Malkin, D., Prykhozhij, S.V., Berman, J.N., tp53 R217H and R242H mutant zebrafish exhibit dysfunctional p53 hallmarks and recapitulate Li-Fraumeni syndrome phenotypes, 167612167612, Copyright (2024) with permission from Elsevier. Full text @ BBA Molecular Basis of Disease