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Fig. 3 tp53 zebrafish mutant embryos exhibit partial-to-full resistance to apoptosis and point mutants exhibit dominant negative activity following induction of the DNA damage pathway. (A) Embryos were exposed to ±30 Gy of IR at 24 hpf and stained with AO at 6 hpi. (B) 26 hpf embryos were treated with 0.1 μM CPT or DMSO for 4 h and stained with AO at 30 hpf. The white arrows highlight the typical apoptotic pattern observed along the dorsal spine. Images are of the dorsal spine above the yolk extension. Representative images shown for each group as described by the ratio in bottom right corner of each image. n = 3 replicates. Scale bars, 100 μm. (C, D) Bar graphs showing quantification of the relative area of apoptotic cells in the dorsal neural progenitor region as determined using an ilastik Cell Profiler pixel classifier for the homozygotes (C) and heterozygotes (D). The number of embryos used for analysis in each group is listed in (A) for (C) and in (B) for (D). Statistical significance was determined by a two-way ANOVA and a Tukey's Honest Significant Difference test as shown by stars. Error bars represent standard error. The Y-axis scale is square root.
Reprinted from Biochimica et biophysica acta. Molecular basis of disease, 1871, Kobar, K., Tuzi, L., Fiene, J.A., Burnley, E., Galpin, K.J.C., Midgen, C., Laverty, B., Subasri, V., Wen, T.T., Hirst, M., Moksa, M., Carles, A., Cao, Q., Shlien, A., Malkin, D., Prykhozhij, S.V., Berman, J.N., tp53 R217H and R242H mutant zebrafish exhibit dysfunctional p53 hallmarks and recapitulate Li-Fraumeni syndrome phenotypes, 167612167612, Copyright (2024) with permission from Elsevier. Full text @ BBA Molecular Basis of Disease