FIGURE 8

FIGURE 8

Principal component analysis, heatmap, and volcano plot showing DEGs for controls and crim1^−/− larvae. Data from DEGs with adjusted P-values </ = 0.05 and an absolute (log2foldchange) >/ = 1 were used to generate the plots. (A) Three-dimensional PCA plot showing the separation of samples from control larval eyes (blue dots) compared to samples from crim1^−/− larval eyes (red dots). (B) Volcano plot showing differential gene expression for crim1^−/− mutant larvae and controls. The log2 fold change indicates the mean expression level for each gene. Each dot represents one gene. Selected genes with an adjusted P-value </ = 0.01 and absolute (log2foldchange) >/ = 2 have been named. Downregulated genes are shown in blue and upregulated genes are shown in red. (C) Heatmap of expressed genes in crim1^−/− larvae and controls, showing separation of gene expression between control and crim1^−/− mutant larvae. The type of sample (control or crim1^−/− mutant) is listed on the X axis and the Y axis contains DEGs.