Fig. 3

Fig. 3

Characterization of IgM antibody titers in zebrafish AGS model treated with tick salivary proteins. Experiment 1. Zebrafish were treated with recombinant tick salivary proteins, metalloprotease (MET) or allergen-like p23 (p23), in combination with saliva non-protein fraction (NPF) and, in comparison with tick saliva, saliva NPF, Subolesin (SUB)-NPF and PBS. (A) IgM antibody titers against tick salivary gland (SG) protein extract. (B) IgM antibody titers against α-Gal. (C) IgM antibody titers against SUB. (D) IgM antibody titers against tick protein p23. (E) IgM antibody titers against tick protein MET. Experiment 2. Zebrafish were treated with a combination (Mix) that included both recombinant tick salivary proteins, MET and p23, with NPF and in comparison, with tick saliva, and PBS. (F) IgM antibody titers against tick SG protein extract. (G) IgM antibody titers against α-Gal. (H) IgM antibody titers against tick protein p23. (I) IgM antibody titers against tick protein MET. Significant differences between treatments and PBS control are shown with post-hoc Pairwise Mann–Whitney p-values (* p < 0.05, ** p < 0.01, *** p < 0.001).