ZFIN Image: Patra et al., 2025, Fig. 8

Image

Figure Caption

Fig. 8 Nephronectin promotes HUVEC migration, tube formation, and stabilization in vitro. A, Cell migration assay: representative brightfield images of wound assays 1 and 6 hours after scratching. Cells were cultured on gelatin? (control) or mr?NPNT?coated 24?well cell culture plates. B, Quantitative analysis of cell migration (n=4). The mean value of the control was considered 100% in each case. C, Tube formation assay: representative brightfield images of HUVECs cultured on Matrigel with BSA (10 ?g/mL, control) or mr?NPNT (10??g/mL) at 4 and 22 hours after seeding. D?F, Quantitative analysis of tube branching (D), and tube stability (E, F) (n=4). Mean value of the control was considered 100% in each case. Statistical significance was determined by a 2?tailed Student's t test. Data are mean�SEM. ns: P>0.05. *P?0.05. HUVEC indicates human umbilical vascular endothelial cell; and mr?NPNT, recombinant mouse nephronectin.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Am. Heart Assoc.