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Fig. 3 Oxymatrine administration promotes thrombopoiesis in Tg (cd41:enhanced green fluorescent protein (eGFP)) transgenic zebrafish. (A) Cd41:eGFP+ thrombocytes in the caudal hematopoietic tissue (CHT) region of oxymatrine (10, 20, and 40 μM) treated and control zebrafish. (B) Quantification of cd41:eGFP+ cells in the CHT region in each group (n = 4 per group). (C) Flow cytometry analysis of cd41:eGFP+ cells in zebrafish embryos. (D) Quantification of cd41:eGFP+ cells in the zebrafish embryos in each group (n = 3 per group). Data represent the mean ± standard deviation (SD) of four or three independent experiments and are analyzed by one-way analysis of variance (ANOVA) with Dunnett's 154. ∗∗P < 0.01 and ∗∗∗P < 0.001 vs. the control. SSC: side scatter; FITC: fluorescein isothiocyanate