Fig. 1

Fig. 1 Pharyngeal ectoderm derived Wnt2bb is required for pharyngeal cartilage development. A: Bright-field images of WT and wnt2bb?/? embryos at 5 dpf. Numbers indicate the number of embryos with corresponding phenotypes out of the total number of embryos examined. B: Pharyngeal cartilages stained with Alcian blue in WT and wnt2bb?/? embryos at 5 dpf. C: Individual cartilage elements stained with Alcian blue in WT and wnt2bb?/? embryos at 5 dpf. D: Quantification of cartilage area in WT and wnt2bb?/? embryos (n = 3). E: Quantification of cartilage length in WT and wnt2bb?/? embryos (n = 5). F: WISH experiments showing the expression of wnt2bb in the pharyngeal region of WT embryos at the indicated stages. The dotted lines on the bottom panels refer to the positions of the corresponding sections. G: The expression pattern of wnt2bb in WT embryos with a Tg(fli1:EGFP) background. Embryos were subjected to a FISH experiment with a wnt2bb probe followed by immunofluorescence staining using the anti-GFP antibody. H: A schematic showing that wnt2bb is derived from the pharyngeal ectoderm and is expressed right next to the neural crest cells which subsequently differentiate into chondrocytes. ?, P < 0.05; ??, P < 0.01; ???, P < 0.001 (student's t-test). WT, wild-type; m, Meckel's cartilage; pq, palatoquadrate; hs, hyosymplectic; ch, ceratohyal; cbs, ceratobranchial cartilages; PA, pharyngeal arch; CNCC, cranial neural crest cell; dpf, days post fertilization. Scale bars, 100 ?m (A?C, F); 20 ?m (G).