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Fig. 5 Wnt2bb controls pharyngeal chondrocyte maturation by activating ?-catenin dependent signaling pathway. A and B: wnt2bb?/? embryos with a Tg(fli1:EGFP) background were treated with 10 ?M BIO or 10 ?M HLY78 from 28 hpf, and collected for Alcian blue staining (A) and live imaging (B) at 5 dpf, respectively. 3D reconstruction of chondrocytes (B) in ceratohyal cartilages was performed using Imaris software. The dotted lines and asterisks represent individual cells subjected to in-depth analysis via 3D reconstruction. C: The volume of individual chondrocytes in ceratohyal cartilages was quantified via Imaris (n = 7). ???, P < 0.001; ns, not significant (student's t-test). WT, wild-type; m, Meckel's cartilage; ep, ethmoid plate; pq, palatoquadrate; hs, hyosymplectic; ch, ceratohyal; cbs, ceratobranchial cartilages; dpf, days post fertilization. Scale bars, 100 ?m (A); 20 ?m (B).
Reprinted from Journal of genetics and genomics = Yi chuan xue bao, , Guo, X., Yang, L., Wang, Y., Yuan, M., Zhang, W., He, X., Wang, Q., Wnt2bb signaling promotes pharyngeal chondrogenic precursor proliferation and chondrocyte maturation by activating Yap expression in zebrafish, , Copyright (2024) with permission from Elsevier. Full text @ J. Genet. Genomics