|
Fig. 4 Wnt2bb promotes chondrocyte precursor proliferation by activating ?-catenin dependent signaling pathway. A and B: Lateral-viewed immunofluorescence images displaying ?-catenin levels in WT and wnt2bb?/? embryos with a Tg(fli1:EGFP) background at 36 hpf. Embryos were fixed and stained with anti-?-catenin and anti-GFP antibodies. Nuclei were labeled with DAPI. The dotted lines represent the magnified area of PA2 in (A). C: The relative fluorescence intensity of nuclear ?-catenin in PA2 (n = 8). D: The relative fluorescence intensity of nuclear ?-catenin in PA4 of WT (n = 5) and wnt2bb?/? (n = 6) embryos. E: Expression of cyclin D1 in WT and wnt2bb?/? embryos at 3 dpf. Numbers at the bottom right corner indicate the number of embryos with phenotypes shown out of the total number of embryos examined. F and G: wnt2bb?/? embryos with a Tg(fli1:EGFP) background were treated with 10 ?M BIO or 10 ?M HLY78 from 28 hpf, and harvested for cell proliferation assay at 36 hpf (F). Virtual puncta for BrdU incorporated cells in PA1 and PA2 were produced via Imaris software. The number of BrdU incorporated chondroprogenitors was quantified in G (n = 4). ???, P < 0.001; ns, not significant (student's t-test). WT, wild-type; PA, pharyngeal arch; hpf, hours post fertilization; dpf, days post fertilization. Scale bars, 20 ?m (A, B, F); 100 ?m (E).
Reprinted from Journal of genetics and genomics = Yi chuan xue bao, , Guo, X., Yang, L., Wang, Y., Yuan, M., Zhang, W., He, X., Wang, Q., Wnt2bb signaling promotes pharyngeal chondrogenic precursor proliferation and chondrocyte maturation by activating Yap expression in zebrafish, , Copyright (2024) with permission from Elsevier. Full text @ J. Genet. Genomics