Figure 9—figure supplement 1.

Figure 9—figure supplement 1.

(A) Signal-to-noise ratio (SNR) of mEPSPs in electrophysiology and ASAP5 for five neurons. (B) Overlay of simultaneous current-clamp and ASAP5 recording. Events detected by miniML in both types of recordings are indicated. (C) Examples of mEPSPs detected in the electrophysiology data that were either detected by miniML in ASAP5 data (orange dots) or missed (blacked dots). (D) mEPSP amplitude versus optical amplitude for detected events in five neurons (color-coded). Line is a linear fit to the data (slope = 0.95 mV/−%ΔF/F0, Pearson correlation coefficient = 0.83). (E) Event half decay time (Left) and rise time (Right) for five neurons from electrophysiology (’Ephys’) and ASAP5 data. Data were calculated from the event averages of each cell. The lower sampling rate of imaging acquisition (400 Hz) vs. electrophysiology (10,000 Hz) likely contributes to the slower event kinetics observed in ASAP5 data.