ZFIN Image: O'Neill et al., 2025, Figure 8.

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Figure Caption

Figure 8. Optical detection of spontaneous glutamate release events in cultured neurons using iGluSnFR3 and miniML.

(A) miniML was applied to recordings from rat primary culture neurons expressing iGluSnFR3. Data from Aggarwal et al., 2023. (B) Example epifluorescence image of iGluSnFR3-expressing cultured neurons. Image shows a single frame with three example regions of interest (ROIs) indicated. (C) ΔF/F0 traces for the regions shown in (B). Orange circles indicate detected optical minis. Note the different signal-to-noise ratios of the examples. (D) Top: Heatmap showing average optical minis for all sites with detected events, sorted by amplitude. Bottom: Grand average optical mini. (E) Top: Histogram with kernel density estimate (solid line) of event amplitudes for n = 1524 ROIs of the example in (B). Note the log-scale of the abscissa. Bottom: Event frequency histogram.

Acknowledgments

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