Figure 1.

Figure 1.

High glucose treatment induced the aggregation of vascular endothelial cell (EC) nuclei in the intersegmental vessels (ISVs) of zebrafish embryos. A and B, Schematic diagram showing the high glucose treatment timeline and confocal imaging region. C through G, Confocal imaging analysis of control (Ctrl) and high glucose–treated Tg(fli1a:nGFP::kdrl:ras-mCherry) embryos at 72 hours post-fertilization (hpf). C′ through G′, The magnifications of the red dotted boxes in C through G, respectively. The arrowheads indicate the EC nuclei in the ISV. I, The distance from the EC nuclei to the midline (DNTM) in ISVs in Ctrl and high glucose–treated embryos. J, The proportion of EC nuclei in ISVs in the 4 zones is shown in E and H. K, Statistics of the nearest neighbor distance (NND) of EC nuclei in Ctrl embryos (n=16) and high glucose–treated embryos (n=19). t test. L and M, Statistics of the range and SD of DNTM in Ctrl embryos (n=15) and high glucose–treated embryos (n=18). t test. Scale bars, 100 µm.