Fig. 2.

Fig. 2.

Maximal pressure created in alternate configurations of the larval zebrafish TBI model. To optimise TBI forces, four equipment parameters were adjusted in concert: (1) a 10 ml or 20 ml syringe was used, and (2) the syringe was held by either a clamp or a rigid foam block. Next, (3) weights of various masses were dropped (4) from various heights (108 cm, 54 cm, 27 cm). Weights ranging from 65-300 g were dropped onto the syringe, and the pressure levels generated for each configuration were measured using Arduino IDE software. Pressure values displayed are the mean values of the maximal pressure reading achieved from three independent weight drop recordings. (A,B) Example time course of pressure dynamics and average pressure generated per pressure wave in the 108 cm/20 ml syringe/foam block TBI setup. These data are duplicated and expanded in Figs. S2-S5 to highlight that additional pressure waves are generated due to the weight bouncing on impact. (C-F) Maximal pressure measurements for each indicated parameter combination. Maximal pressure can be manipulated across three orders of magnitude through changing various TBI assay settings. Alterations to the mass of the weight dropped had the largest impact on maximal pressure, followed by changes to the drop height of the weight. Using a foam block to hold the syringe and decreasing the syringe size both modestly increased maximal pressure.