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Fig. 1 The intercellular domain (ICD) of zebrafish Jagged2 interacts with the N-terminal domain (NTD) of PEX1. (A, B) Schematic diagrams of the constructs used to yeast-two hybrid screening and Western blotting. (A) Schematics of Myc-tagged Jagged2 (J2) constructs. (B) Schematics of PEX1. An identical 516 bp-long fragment of PEX1 called PEX1Y2H isolated from yeast-two hybrid screening using J2ICD as the bait. (C) Co-immunoprecipitation (Co-IP) performed using the lysates of NIH 3T3 cells transfected with expression vectors for Myc-tagged J2 variants and Flag-tagged PEX1Y2H, as indicated. Immunoprecipitated with and anti-Myc antibody and separated by SDS-PAGE followed by transfer to a PVDF membrane and immunoblotting with anti-Flag antibody. Myc-tagged RFP used as a control for Co-IP. Antibody for anti-α-tubulin used as an internal control. SP, signal peptide domain; DSL, Delta/Serrate/LAG-2 domain; CR, cysteine-rich domain; TM, transmembrane domain; AAA, AAA-ATPase domain.