ZFIN Image: Cerveró-Varona et al., 2025, Figure 8

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Figure Caption

Figure 8

Exposure of injured zebrafish larvae to AEC-derived MV stimulated early immune cell recruitment

(A) Illustration depicting the experimental plan followed for the treatment of 72 hpf zebrafish larvae with AEC ± LPS_MV and subsequent time lapse observation.

(B) Exemplary images of Tg(lysz:dsRed2) larvae post tail fin resection, showcasing the localization of immune cells in the tail and their accumulation at the site of injury from 0.5 to 6 hpd.

(C) Densitometric analysis of RFU, representing the amounts of immune cells detected in the total area and within the wounded area, normalized over CTR (black line). Data (mean ± SD) represent 3 independent sets of experiments (n = at least 3 biological replicates in each group per set). ∗, ∗∗ Statistically significant values between the different studied groups (p < 0.05, p < 0.01, respectively). One-way ANOVA was employed for comparing normally distributed data. Scale bar, 100 μm.

Acknowledgments

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