Fig. 3

Fig. 3

Knockdown of SNHG4 decreases the growth and metastasis of glioma cells in zebrafish xenograft. a The glioma cells transfected with si-SNHG4 or NC were injected into the PVS of 2-dpf (days post‑fertilization) wild type zebrafish larvae. Images of the yolk were taken by a stereomicroscope at 4 dpi. b The CM-DiI-positive areas in the yolk were quantified for cell proliferation. c The typical images were imaged by confocal microscope. d The glioma cells transfected with si-SNHG4 or NC were injected into the PVS of 2-dpf wild type zebrafish larvae. Images of the trunk were taken by a stereomicroscope at 4 dpi. e The CM-DiI-positive areas in the trunk were quantified for cell metastasis. f The typical images were imaged by confocal microscope. g, h Statistical analysis of proliferation (g) and metastasis (h) when knocking down SNHG4 in Ln229 cells (n = 17), compared with NC (n = 16) (P = 0.0001 and 0.0131). i, j Statistical analysis of proliferation (i) and metastasis (j) when knocking down SNHG4 in U251 cells (n = 14), compared with NC (n = 11) (P = 0.0002). *: P < 0.05, ***: P < 0.001. Scale: 100 μm