Fig. 5

Fig. 5

The effect of CRISPR/Cas9-induced mutations in 13 obesity genes on food intake in 8-day-old CRISPR/Cas9 founders. Dots and error bars show effect sizes and 95% confidence intervals for affected larvae (number on the left) vs. controls (number on the right). The effect of being in the first quartile of food intake [Q1]; left); the effect of being in the third quartile (Q3; middle); the effect of being in the top quartile (Q4; right). All are compared with the second quartile (Q2) as a reference. The results for irs2 and lep are derived from the concurrent targeting of both orthologues (i.e., irs2a and irs2b; lepa and lepb). Effects have been adjusted for batch, tank and time of day at imaging. Effects with P < 0.05 are shown in red.