ZFIN Image: Gu et al., 2025, Figure 1

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Figure 1

High-fat diet (HFD)-induced non-alcoholic fatty liver disease (NAFLD) progression involved in glucose and fatty acid metabolism: (A,B) Photographed fish and liver and corresponding Body Mass Index (BMI) and relative liver weight in normal diet (ND) and HFD groups. (C,D) Liver sections by hematoxylin-eosin (HE) and Oil Red O (ORO) staining and their corresponding liver vacuolation density in HE-stained sections and optical density in ORO staining. (E) Biochemical determination on lipid indicators including triglyceride (TG), total cholesterol (T-CHO), free fatty acid (FFA), glucose, and its metabolic intermediate of pyruvic acid. (F) Transcriptional levels of the genes related to glucose metabolism (glycolysis, pyruvic acid dehydrogenation, TCA cycle), and fatty acid metabolism (PPAR pathway transduction, fatty acid synthesis, fatty acid transport, fatty acid esterification, and fatty acid β-oxidation), determined using qRT-PCR. Values are the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control.

Acknowledgments

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