Fig. 2

Fig. 2 Internalization of PS-NPs in male germ cells. Cytometer profiles and enrichment analysis resulting from the sorting of testicular disaggregated samples from both control and labeled PS-NPs exposed individuals. (A) Control and PS-NPs cytometer scatter plot showing positive and negative cells for Dragon-Green fluorescent particles. Scatter plot axes represent B2 detector (B2 (515)-A), to identify positive cells for the fluorochrome, versus side scatter (SSC (Imaging)-A), which organizes cells depending on their complexity. (B) Spectral plot for B2 fluorochrome detector (517 nm) showing the density of the events by intensity for this specific detector in control and PS-NPs samples. (C) Cytometry imaging of the sorted populations, selecting the parameters LightLoss (Imaging) and Dragon-Green fluorochrome detector for both control (negative population) and PS-NPs (positive population) samples. (D) Enrichment analysis bar plot for the sorted populations representing the percentage of each spermatogenic cell type (Sperm; RS: round spermatids; SpcI: spermatocytes I; Spg: spermatogonia) in different populations (Control negative, PS-NPs negative and PS-NPs positive) (Chi-square, *p < 0.05, ***p < 0.001). A total of 120–140 cells were analyzed for each population.