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Fig. 6 - Supplemental 5 Microscale thermophoresis (MST) binding analysis of candidate proteins identified with DC661-H1. (A–C) Thermophoretic movement of DC660 bound to purified target proteins labeled with Cy5 dye. TOM1L2, TOM1, and TRAPPC3 exhibited ligand-induced aggregation, suggesting their interaction with DC660. (D–H) Dose–response curves of normalized fluorescence changes for target proteins binding. VPS29 demonstrated substantial affinity to DC660 with a Kd of 24.95 ± 11.71 µM (D), but showed no binding to HCQ (H). TOM1L2, TOM1, and TRAPPC3 did not show detectable binding to HCQ (E–H). n = 2. Data are shown as mean ± s.d.