Fig. 2

Fig. 2

Visual impairment in the sacs^-/- larval zebrafish. (A-A') Zebrafish sacs^-/- mutant larvae at 120 hpf showed reduced optokinetic response. The frequency was calculated considering the number of saccades per minute for each larva examined (N = 40 for each group, sacs^-/- and controls). (B-B′) Light-Dark preference test was performed according to (Rock et al., 2022). At 120 h post-fertilization, sacs^-/- mutant larvae displayed freezing behavior under light conditions (measured as crossed time per minute) and showed no preference for the bright/white area during the dark phase (measured as percentage of time spent in the light area (N = 15 for each group,sacs^-/-and controls). ****p < 0.0001 was calculated by Kruskal Wallis test multiple comparisons. (C-C′) Zebrafish sacs^-/- mutant larvae at 120 hpf showed an affected visual motor response (VMR). Sacs^-/- mutants motor activity appears significantly higher in light- off conditions compared to controls siblings and greatly reduced in light-on condition (n = 20 for each group, sacs^-/- and controls). ****p < 0.0001 was calculated by two-tailed Mann-Whitney test. Error bars indicate mean ± s.e.m.