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Fig. 5 Zebrafish cardiac cell apoptosis assay with AC16 cells to validate RES mitogenic effect. (A) Zebrafish tunnel staining assay. (B) Tol2 EGFP fluorescent plasmid transfection of AC16 cells. (C) VPH on AC16 cells viability assay. (D) RES on AC16 cells viability assay. (E) VPH combined with RES administration assay to detect the remission effect of RES on cells assay. (*: P < 0.05, **: P < 0.05, ***: P < 0.001, ****: P < 0.0001, NS: not statistically different).
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