ZFIN Image: Ma et al., 2025, Figure 3

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Figure Caption

Figure 3

Characterization of the expression pattern of Tg(3kmuc5AC:EGFP) and Tg(3kmuc5AC:EGFP). (A) Schematic diagram of the Tol2 construct used to generate the Tg(3kmuc5AC:EGFP) reporter. (B,C) Dorsal view and lateral view of a Tg(3kmuc5AC:EGFP) larva at 5 dpf. Scale bars: 200 μm. (D,E) Head and posterior region of operculum shown by Tg(3kmuc5AC:EGFP) in adult zebrafish. White arrows label canal neuromasts. Scale bars in D and E: 1 mm. (F) Schematic diagram of the Tol2 construct used to generate the Tg(3kmuc5AC:H2A-mCherry) reporter. (G–G”) Immunostaining with anti-mCherry (red) and anti-Sox2 (green). (H–H”) Live imaging of Tg(3kmuc5AC:H2A-mCherry) (red) stained with Yo-Pro-1 (green). (I–I”) Live imaging of Tg(3kmuc5AC:H2A-mCherry) (red) and Tg(ET20:EGFP) (green) double transgenic line. Scale bars: 20 μm.

Acknowledgments

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