Fig 4

Fig 4 Zygotic Asz1 is not required for PGC specification and migration.

A.asz1^+/+;Tg(ddx4:GFP) and asz1^-/-;Tg(ddx4:GFP) gonads at 3 wpf, labeld for Ddx4 (magenta) and DAPI (blue), showing similar number of Ddx4+ germ cells. Scale bars are 15 μm. B. The number of Ddx4+ germ cells from A, is plotted. n = number of gonads. Bars are mean ± SD. C. Ddx4 labeling in 24 hpf wt, asz1^+/-, and asz1^-/- embryos shows normal localization of PGCs to the presumptive gonad region (arrowheads). D. The number of PGCs per embryo as shown in C is plotted for each genotype and shows non-significant variation across genotypes. n = number of embryos. Bars are mean ± SD. E. Ddx4 labeling (green) in 5 dpf wt, asz1^+/-, and asz1^-/- larvae. Zoom in images on the presumptive gonad regions are shown. F. The number of PGCs per larvea as shown in E is plotted for each genotype and shows non-significant variation across genotypes. n = number of embryos. Bars are mean ± SD. G. Ddx4 labeling in 7 dpf wt, asz1^+/-, and asz1^-/- larvae shows normal localization of PGCs to the forming gonad region. Bottom images are zoom-in magnifications of the boxes in larvae in the top panels. H. The number of PGCs per larva as shown in G is plotted for each genotype, and shows non-significant variation across genotypes. n = number of embryos. Bars are mean ± SD.