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Fig. 4 Hematotoxicity and liver toxicity of the most potent 4-AQ molecules 6, 11, and 24 compared to HHQ and miconazole.a The hemolytic activity tested on sheep red blood cells was compared with that of HHQ and miconazole, a clinical antifungal drug used as a positive control. b The hemolysis rate was determined in relation to the hemolysis of erythrocytes induced by 1% Triton X, which was arbitrarily set to 100%. Values are average of two independent experiment performed in triplicates ± SD. Statistically significant differences between the treated groups and control (0.1% DMSO) were determined using one-way ANOVA (***P < 0.001). c Hepatotoxicity was evaluated in transgenic Tg(-2,8fabp10:EGFP) zebrafish embryos with fluorescently labeled liver (n = 8) using the liver area index. d The morphology of the transgenic zebrafish embryos and their liver fluorescence after different treatments is shown. One-way ANOVA with the Bonferroni test was used to determine statistical significance by comparing fluorescence signal of the control sample with each treated group (*** P ≤ 0.001).