|
Fig. 2 The 4-AQ anti-virulence compounds have a synergistic interaction with nystatin in the suppression of Candida albicans filamentation.Inhibition of filamentation of C. albicans SC5314 in combined treatment with nystatin and 4-AQ molecules (6, 11 and 24) compared to the individual agents, evaluated in RPMI medium (a, b) and Spider medium (c, d). Color-based heat maps (a, c) show the categorized filamentation phenotypes after the 3-day treatments, assessed by the morphology of the fungal colonies (filament density and length, b, d) in comparison to the control (DMSO) as follows: no effect (very dense and long filaments developed; black boxes), weak inhibitory effect (lower filamentation density with long filaments; dark gray boxes), moderate inhibitory effect (short filaments developed; light gray boxes), good inhibitory effect (rare filaments developed; light green boxes), and complete inhibition (no filaments developed; green boxes).