ZFIN Image: Chen et al., 2024, Figure 6

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Figure Caption

Figure 6

Identification of differential alternative splicing events between WT and ddx4-E4Δ zebrafish ovaries. (A) Five types of alternative splicing events analyzed in this study are shown. (B) The numbers of DASEs for each AS type between WT and ddx4-E4Δ ovaries are shown. (C) RNA-seq tracks of aak1a, ndufv3, and sh3d21 are shown. The alternative exons are indicated with boxes. (D) RT-PCR validation of the three selected DASEs. Original gel images are shown in Figure S1B. (E) The differentially spliced exons affected by ddx4-L knockout have relatively shorter length, higher GC contents, similar 5′ splicing sites, and stronger 3′ splicing sites.

Acknowledgments

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