Figure 4

Figure 4

Effect of podocarpusflavone A (PCFA), isoniazid (INH), and INH-PCFA combinations on Mycobacterium tuberculosis H37Rv strain growth in THP-1-derived macrophages. (A) THP-1 macrophages were infected and treated with PCFA at 32, 64, and 128 µM and INH at 0.5× MIC (0.032 µg/mL) and 1× MIC (0.063 µg/mL). (B) THP-1 macrophages infected and treated with combinations of INH-PCFA. The intracellular viability of the bacteria was determined by CFU counting. Values were reported as mean ± error. The mean value of each group was significantly different from the mean value of the controls, as indicated in the graph after the two-way ANOVA analysis. *** p < 0.001, ** p < 0.01, and * p < 0.05 compared to no drug. ^### p < 0.001 and ^## p < 0.01 compared to INH 0.5× MIC. ⁺⁺⁺ p < 0.001 and ⁺ p < 0.05 compared to INH 1× MIC.