Figure 3

Figure 3

Growth inhibition of Mycobacterium strains by podocarpusflavone A (PCFA). Microplate assay after 5 days of incubation in the presence of PCFA (16–128 µM). Growth of Mycobacterium tuberculosis strains H37Rv and M299 was evaluated by the MTT test. The growth of M. marinum E11 mCherry red-fluorescent bacteria was assessed by measuring bacterial fluorescence (excitation at 588 nm and emission at 610 nm). The untreated Mycobacterium strains were used as a negative control, and rifampicin (RIF) was used as a positive control, with MIC values for each of the strains equal to 0.3 µg/mL for M299; 1 µg/mL for H37Rv and 4 µg/mL for E11 mCherry, respectively.