Fig. 1.

Fig. 1.

The insertional smb mutant presents median fin phenotypes. (A) Whole mount wild-type adult (n=50) and heterozygous smb^{Tg(sox10:Gal4)co3021} (n=50) zebrafish (hereafter smb mutants) were live imaged with transmitted light. In all panels, asterisk denotes the missing dorsal fin; caret indicates reduced size anal fin. (B) Schematic indicating the transgenic sequence and genomic location of the sox10:Gal4 insertion in the smb background. (C) Wild-type and smb heterozygous mutant juveniles (33 dpf) were stained with Alcian Blue (cartilage) and Alizarin Red (bone) and imaged by light microscopy. (D) Enlargements from panel C depicting the radials and rays of the dorsal and anal fins in wild types and mutants. Arrowhead indicates disorganized anal fin radials in mutants. (E) Representative smb heterozygous mutant with partial fin loss was imaged with transmitted light. (F) Arrowheads in enlargements indicate fused radials in reduced dorsal and anal fins. Scale bars: 2 mm (A); 1 mm (C,E).