Fig 4

Fig 4 Comparative analysis of the control and ctu1 morphant across three main embryonic germ layers.

(A) UMAP visualization of three germ layers. (B) UMAP visualization of key marker genes expression. Color scale represents log-normalized expression. The marker genes for neural cells are elavl3, sox19a, rx1; for mesodermal cells, the markers are pmp22a and alas2; and for e pidermal cells, the marker gene is epcam. (C) The bar plot shows signal pathways affected by ctu1 deficiency in epidermal, mesodermal, and neural cells as identified by GSEA. All terms demonstrate significant enriched (adj.p < 0.05) and normalized enrichment scores (NES) are shown. (D) RNA velocity plot of control and ctu1 morphant mesodermal cells.