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Fig. 6 FA supplementation affects the expression of proteins and genes in the MAPK signaling pathway in the presence of BPA. (A) Western blot analysis was performed to detect the expression of MAPK signaling pathway activation-related proteins in 5 dpf zebrafish larvae following exposure to BPA and supplementation with FA. The results of statistical analysis are shown in (B-G). Additionally, mRNA expression of relevant genes in the MAPK signaling pathway was verified using qRT-PCR in (H). The data is shown as mean ± standard error of three replicates (n = 50), with significance levels denoted as *P<0.05, **P<0.01, ***P<0.001, and ***P<0.0001.