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Fig. 2 The addition of FA can improve the abnormal movement behaviors of zebrafish embryos or larvae induced by exposure to BPA. (A) A stereo fluorescence microscope was used to record a video of the tail oscillatory movement of zebrafish embryos at 24 hpf. The sequence of pictures shows intervals of 30 seconds. (B) The frequency of tail oscillation was calculated for the 24 hpf embryos. (C) The movement trajectory, (D) total movement distance and (E) average speed of 6dpf larvae within a 10-minute period were also analyzed. Significance levels are denoted as follows: *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001. Error bars represent the standard error of the mean.