Fig. 3.

Fig. 3.

myh^−/− mutant phenotypes between 24 hpf and 5 dpf. a–e) Representative bright-field images of myh9a^−/−, myh9b^−/−, and myh10^−/− homozygous mutants compared to WT at 24 hpf, 48 hpf, 72 hpf, 96 hpf, and 5 dpf. Arrowheads indicate location of pericardial edema in myh9b^−/− mutants. f) Quantification of myh9b pericardial edema phenotype development and reversal from 48 hpf to 6 dpf in offspring generated from an myh9b^+/− parent incross. The χ² analyses determined that at 72 hpf the edema phenotype development is Mendelian, and at 96 hpf and beyond it is no longer Mendelian. Scale bars for all images = 0.5 mm. n = 610 for phenotype development and reversal quantification in f.