Fig. 4

Fig. 4

Western blot analysis of glucose transporters (GLUTs). A and B: GLUT expression from whole zebrafish embryos (3 dpf) and from head, and body, including the yolk sac. The expression of each GLUT and respective β-actin on the membrane is shown on A (top GLUT, bottom β-actin). B: quantification of the relative expression of each GLUT normalized by β-actin. C: GLUT protein level from zebrafish embryos (3 dpf) normalized to β-actin, after exposure to fractions 06104_D and 03283_B at 10 µg/ml and Emodin at 10 µM, using DMSO (0.1%) as solvent control. Three replicates consisting of a pool of 16 larvae each were used per condition in two independent assays (n = 6). The data is represented as box-whisker plots from the fifth to 95th percentiles, and asterisks highlight significant differences vs. the solvent control (*** p < 0.001; ** p < 0.01; * p < 0.05), One-Way ANOVA and Dunnett’s posthoc test.