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Fig. 7 Neuroprotective effects of coumestrol against oligomeric amyloid-? (A?)-induced cytotoxicity. BV2 mouse microglia were pretreated with coumestrol and exposed to oligomeric A?. (A) After 24 h, BV2 cell viability was measured by MTT assay. (Coumestrol, n = 6; N-acetyl cysteine (NAC), n = 2) (B) Reactive oxygen species (ROS) levels were assessed by flow cytometry with H2DCFDA staining. Coumestrol pretreatment significantly restored BV2 cell viability and reduced ROS levels. All results are expressed as the mean ± SEM. Statistical significance was determined by Kruskal-Wallis test followed by Dunn's post-hoc test (* p < 0.05, ** p < 0.01, *** p < 0.005).
Reprinted from International journal of biological macromolecules, 282(Pt 4), Peng, C.H., Hwang, T.L., Hung, S.C., Tu, H.J., Tseng, Y.T., Lin, T.E., Lee, C.C., Tseng, Y.C., Ko, C.Y., Yen, S.C., Hsu, K.C., Pan, S.L., HuangFu, W.C., Identification, biological evaluation, and crystallographic analysis of coumestrol as a novel dual-specificity tyrosine-phosphorylation-regulated kinase 1A inhibitor, 136860, Copyright (2024) with permission from Elsevier. Full text @ Int. J. Biol. Macromol.