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Fig. 5 Rho A inactivation contributes to the R1881-induced Golgi body reorientation in HUVECs. (a) Treatment with R1881 (5 nM) for 6 h induced the Golgi apparatus to move away from the nucleus, and this effect was abolished by pre-treatment with HF (5 nM). The cells were fixed with methanol, and then labeled with an anti-TGN 38 antibody followed by a FITC-conjugated secondary antibody. The nuclei were visualized with DAPI staining. The TGN 38 positive immunoreactivity (green color), which is a Golgi apparatus marker, was indicated by the arrow. Bar = 25 μm. (b) The transmission electron microscope (TEM) images track the location of the Golgi apparatus in control (top panel) and R1881-treated HUVECs (bottom panel). Bar = 10 μm. (c) Treatment with R1881 (5 nM) for 6 h caused the Golgi apparatus to move away from the nucleus in HUVECs, and this effect was abolished by transfection with RhoA V14 pcDNA. However, this prevention effect induced by RhoA V14 pcDNA transfection was abolished by pre-treatment with Y27632, a ROCK inhibitor. The cells were fixed with methanol, and then labeled with an anti-TGN 38 antibody followed by a FITC-conjugated secondary antibody. The nuclei were visualized with DAPI staining. The TGN 38 positive immunoreactivity (green color) was indicated by the arrow. Bar = 25 μm.